Optical biosensor for biogenic amines detection used a porous silicon matrix

J. A. Contreras Rozo, F. Severiano Carrillo, V. L. Gayou, H. Martínez Gutierrez, R. Delgado Macuil

Abstract


The content of biogenic amines has been studied due to the toxicity of these compounds in humans when are consumed exogenously, and is used as an indicator of quality in the food industry. The manly method for its determination is high performance liquid chromatography. However, it requires a long time for analysis. An alternative method is the use of biosensors, porous silicon with gold nanoparticles were obtained by electrochemical attack assisted with metal salt. These substrates were used for the construction of a biosensor capable of detecting BA using diamine oxidase enzyme as an element of biological recognition and is binding using the self-assembled monolayers method. The correlation between scanning electron microscopy, X-ray dispersive energy spectroscopy, and Fourier Transform infrared spectroscopy analysis, demonstrated the correct construction of the biosensor and the detection of biogenic amines by interaction with the enzyme. Finally, with the built biosensor it was possible to detect three important biogenic amines found in food.


Keywords


Biogenic amine, biosensor, self-assembled monolayers, porous silicon

Full Text:

PDF

Refbacks

  • There are currently no refbacks.


Suplemento de la Revista Mexicana de Física

 ISSN: In Process

  Online non-periodical journal published by Socie­dad Mexicana de Física, A. C.

Departamento de Física, 2o. Piso, Facultad de Ciencias, UNAM.
Circuito Exterior s/n, Ciudad Universitaria. C. P. 04510 Ciudad de México.
Apartado Postal 70-348, Coyoacán, 04511 Ciudad de México.
Tel/Fax: (52-55) 5622-4946, (52-55) 5622-4840

e-mail:  rmf@ciencias.unam.mx

https://rmf.smf.mx/ojs/rmf-s