Transfección de células por medio de ondas de choque

E. Armenta Ruiz, A. Varela Echavarría, G. Martínez de la Escalera, A.M. Loske Mehling

Abstract


Treatments for inherited and acquired diseases are pointing towards gene therapy, which depends on methods to introduce foreign genetic material into cells. Unfortunately, most of the methods available to allow incorporation of transgenes into the genome are not selective to target a particular population of cells. The present article describes the use of shock waves as a cell -- permeabilization method to load a reporter gene into cultured cells. Shock waves were generated by underwater electrical breakdown and focused on the vials containing cell suspension using an ellipsoidal reflector. Transfection was achieved due to acoustic cavitation generated inside the test vials after passage of each shock wave. The number of transfected cells was still low; nevertheless, our results show the feasibility of cell transfection by underwater shock waves.

Keywords


Shock waves; acoustic cavitation; transfection

Full Text:

PDF

Refbacks

  • There are currently no refbacks.


REVISTA MEXICANA DE FÍSICA, year 67, issue 1, January-February 2021. Bimonthly Journal published by Sociedad Mexicana de Física, A. C. Departamento de Física, 2º Piso, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, Alcaldía Coyacán, C.P. 04510 , Ciudad de México. Apartado Postal 70-348. Tel. (+52)55-5622-4946, https://rmf.smf.mx/ojs/rmf, e-mail: rmf@ciencias.unam.mx. Chief Editor: José Alejandro Ayala Mercado. INDAUTOR Certificate of Reserve: 04-2019-080216404400-203, ISSN: 2683-2224 (on line), 0035-001X (print), both granted by Instituto Nacional del Derecho de Autor. Responsible for the last update of this issue, Technical Staff of Sociedad Mexicana de Física, A. C., Fís. Efraín Garrido Román, 2º. Piso, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, Alcaldía Coyacán, C.P. 04510 , Ciudad de México. Date of last modification, January 4, 2021.

The responsibility of the materials published in Revista Mexicana de Física rests solely with their authors and their content does not necessarily reflect the criteria of the Editorial Committee or the Sociedad Mexicana de Física. The total or partial reproduction of the texts hereby published is authorized as long as the complete source and the electronic address of the publications are cited.

There is no fee for article processing, submission or publication.

Revista Mexicana de Física by Sociedad Mexicana de Física, A. C. is distributed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License