Dos configuraciones diferentes para la descripción temporal de actividad de Escherichia coli mediante speckle dinámico

E.E. Ramírez Miquet, L. Martí López, O.R. Contreras Alarcón


We present the experimental results of applying the laser dynamic speckle technique to describe bacterial cells activity corresponding to the species Escherichia coli (ATCC 25922) cultivated in two-compartment Petri dishes, using one of them as a non-inoculated control blank. In the experimental series we registered speckled images and after processing them, we obtained optical signals of the bacterial activity corresponding to a single cell concentration and two different laser beam impact configurations. The results for both configurations are discussed. We conclude that the method, the designed and mounted set-up and the associated image processing allowed the detection and description of the bacterial activity.


Dynamic speckle; bacterial activity; inoculum; CFU; image processing

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Revista Mexicana de Física

ISSN: 2683-2224 (on line), 0035-001X (print)

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