Optical biosensor for biogenic amines detection used a porous silicon matrix

Authors

  • J. A. Contreras Rozo Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional
  • F. Severiano Carrillo CONACYT
  • V. L. Gayou Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional
  • H. Martínez Gutierrez CNMN-IPN
  • R. Delgado Macuil Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional

DOI:

https://doi.org/10.31349/SuplRevMexFis.1.3.49

Keywords:

Biogenic amine, biosensor, self-assembled monolayers, porous silicon

Abstract

The content of biogenic amines has been studied due to the toxicity of these compounds in humans when are consumed exogenously, and is used as an indicator of quality in the food industry. The manly method for its determination is high performance liquid chromatography. However, it requires a long time for analysis. An alternative method is the use of biosensors, porous silicon with gold nanoparticles were obtained by electrochemical attack assisted with metal salt. These substrates were used for the construction of a biosensor capable of detecting BA using diamine oxidase enzyme as an element of biological recognition and is binding using the self-assembled monolayers method. The correlation between scanning electron microscopy, X-ray dispersive energy spectroscopy, and Fourier Transform infrared spectroscopy analysis, demonstrated the correct construction of the biosensor and the detection of biogenic amines by interaction with the enzyme. Finally, with the built biosensor it was possible to detect three important biogenic amines found in food.

Author Biographies

J. A. Contreras Rozo, Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional

Postgraduate student Nanobiotechnology

F. Severiano Carrillo, CONACYT

Professor Nanobiotechnology

V. L. Gayou, Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional

Professor Nanobiotechnology

H. Martínez Gutierrez, CNMN-IPN

Professor

R. Delgado Macuil, Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional

Professor Nanobiotechnology

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Published

2020-08-22

How to Cite

1.
Contreras Rozo JA, Severiano Carrillo F, Gayou VL, Martínez Gutierrez H, Delgado Macuil R. Optical biosensor for biogenic amines detection used a porous silicon matrix. Supl. Rev. Mex. Fis. [Internet]. 2020 Aug. 22 [cited 2024 Mar. 28];1(3):49-54. Available from: https://rmf.smf.mx/ojs/index.php/rmf-s/article/view/4897