Design, construction and robust validation of a germicidal device based on UV irradiation: a necessity for hospital disinfection in the COVID-19 era


  • Ismael Martínez-Ramírez Escuela Superior de Física y Matemáticas del Instituto Politécnico Nacional
  • Clemente Cruz-Cruz Hospital Juárez de México
  • Adolfo López Ornelas Hospital Juárez de México
  • Emilio Mariano Duran-Manuel Hospital Juárez de México
  • Enrique Estudillo Instituto Nacional de Neurología y Neurocirugía “Manuel Velasco Suárez”
  • Iván Velasco Instituto Nacional de Neurología y Neurocirugía “Manuel Velasco Suárez”
  • Miguel Angel Loyola-Cruz Hospital Juárez de México
  • Patricia Gutiérrez Zayas-Bazán Escuela Superior de Física y Matemáticas del Instituto Politécnico Nacional
  • Jesús López López-Vargas Escuela Superior de Física y Matemáticas del Instituto Politécnico Nacional
  • Yesenia Godínez-Cruz Escuela Superior de Física y Matemáticas del Instituto Politécnico Nacional
  • Miguel Tufiño Velázquez Escuela Superior de Física y Matemáticas del Instituto Politécnico Nacional
  • Gabriela Ibáñez Cervantes Hospital Juárez de México
  • Juan Manuel Bello López Hospital Juárez de México
  • Gerardo Silverio Contreras-Puente Escuela Superior de Física y Matemáticas del Instituto Politécnico Nacional



UV light; SARS-CoV-2; ESKAPE bacteria; fungi; disinfection


Pandemic by SARS-CoV-2 has revealed the importance of disinfection methods due to pathogens of medical importance being detectable and infective after several hours on contaminated surfaces, including medical devices. The aim of this work was to design, construction, and validation of a UVC light irradiation system in the short wavelength region (200 to 320 nm). We studied the effective of the system through in vitro disinfection to eliminate pathogens such as SARS-CoV-2, ESKAPE bacteria and fungi in biofilm and planktonic forms. Doses of 0.25 J/cm2 (10 s of exposure to UVC light), 100% death of ESKAPE bacteria and fungi in planktonic form was observed. Through biofilm formation induction assays of these microorganisms showed resistance to treatment with UV light; however, their viability was not detected after 20 s of exposure (via confocal microscopy). For SARS-CoV-2, 100% reduction was reached after 120 s of exposure. This evidence shows the need to employ emerging methods of disinfection of surfaces and medical devices since these are potential vehicles for transmitting pathogens. The advantages of using UV light as an emergent disinfection method in the era of COVID-19 are discussed.


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How to Cite

I. Martínez-Ramírez, “Design, construction and robust validation of a germicidal device based on UV irradiation: a necessity for hospital disinfection in the COVID-19 era”, Rev. Mex. Fís., vol. 70, no. 1 Jan-Feb, pp. 010901 1–, Jan. 2024.